Test your knowledge on amino acids and proteins
1) Which of the following amino acids is the smallest amino acid possible?
2) Which three amino acids are essential amino acids?
A. Arginine, Leucine, Alanine
B. Histidine, Lysine, Valine
C. Cysteine, Proline, Tryptophan
D. Methionine, Phenylalanine, Asparagine
E. Serine, Isoleucine, Threonine
3) Which test is used to test for amino acids?
A. Benedict’s Test
B. Biuret Test
C. Iodine Test
D. Ninhydrin Test
E. Molisch’s Test
4) By what process is Cystine formed as shown in the diagram?
5) What kind of bond is shown in the red circle on the diagram?
A. covalent bond
B. disulphide bond
C. ionic bond
D. polar covalent bond
E. metallic bond
6) What is the name of the bond highlighted in blue in the diagram?
A. disulphide bond
B. peptide bond
C. metallic bond
D. ionic bond
E. hydrogen bond
7) What is the name given to a molecule containing three amino acids joined by peptide bonds?
8) The primary level of structure in a protein is:
A. the regular folding of regions of the polypeptide chain.
B. the spatial arrangement of amino acids that are far apart in the linear sequence.
C. the composition and linear sequence of amino acids as joined together by peptide bonds.
D. the first level of the proteins structure
E. the alpha and beta folding of the protein structure
9) What bonds are involved in stabilizing the tertiary structure of a protein?
A. Hydrogen bonds and covalent disulphide bonds
B. Hydrogen bonds, hydrophobic forces, electrostatic forces, covalent disulphide bonds
C. Hydrogen bonds
D. Ionic bonds and covalent disulphide bonds
E. Hydrogen bonds, ionic bonds and electrostatic forces
10) Name the chaotrope in the experiment below and state the role of the chaotrope.
A. Mercaptoethanol; disrupts the hydrogen bonds in the protein structure
B. Urea; disrupts the hydrogen bonds in the protein structure
C. Mercaptoethanol; disrupts the hydrophobic interactions in the protein structure
D. Urea; disrupts the hydrophobic interaction in the protein structure
E. Mercaptoethanol; reduces the disulphide bond in the protein structure
Test your knowledge on Glycolysis
Select the correct multiple answer using ONE of the keys A, B, C, D or E as follows:
A. 1, 2 and 3 are correct
B. 1 and 3 are correct
C. 2 and 4 are correct
D. only 4 is correct
E. all are correct
Question 1: In the energy investment phase of glycolysis:
- There are 2 irreversible reactions
- There are 3 reversible reactions
- Phosphorylation of glucose occurs
- 4 ATP is used
Question 2: The purpose of converting pyruvate to lactate is:
- To regenerate ATP
- To regenerate ATP and NAD+
- To regenerate NADH
- To regenerate NAD+
Question 3: In the conversion of pyruvate to lactate:
- NADH is a cofactor
- The enzyme used in the reaction is lactate decarboxylase
- The reaction is reversible
- ATP is a cofactor
Question 4: In the conversion of pyruvate to Acetyl-CoA:
- TPP is a cofactor
- Lipoate is a cofactor
- FAD is a cofactor
- FADH is a cofactor
Question 5: In the energy generation phase of glycolysis:
- There are 2 irreversible reactions
- Therese are 4 reversible reactions
- 4 ATP used
- 2 NAD+ used
Question 6: In the payoff phase of glycolysis, which 5 enzymes catalyse the 5 reactions?
- Glyceraldehyde 3-phos[ate dehydrogenase
- Pyruvate kinase
- Phosphoglycerate mutase
- Phosphoglycerate kinase
Question 7: Which enzyme(s) catalyses the conversion of acetaldehyde to ethanol?
- Pruvate decarboxylase
- Alcohol decarboxylase
- Pyruvate dehydrogenase
- Alcohol dehydrogenase
Question 8: For every glucose molecule entering glycolysis:
- 2 ATP are used
- 4 NAD+ used
- 4 ATP generated
- 4 NADH generated
Question 9: The net gain of glycolysis is:
- 2 ATP
- 4 NADH
- 2 NAD+
- 2 NADH
Question 10: The two ATP forming reactions (substrate level phosphorylation) are:
- Glyceraldehyde-3-phosphate> 1,3-Bisphosphoglycerate
- 3-phosphoglycerate> 2-phosphoglycerate
- Phosphoenolpyruvate> pyruvate